CDD Vault Update: Normalize Dose-Response Data? Yes We Can!
With this highly anticipated release, you can start importing raw data for your dose-response experiments, and let CDD calculate % activity based on controls. This is building on existing functionality of calculating EC50 based on % activity. We hope you will be able to import dose-response data into CDD as they are generated, eliminating the need for an intermediate processing step.
The options to normalize raw data can be found in the dose-response readout definition section of the protocol:
- Normalize within each plate: Controls are present on each tested plate. Data will be normalized per plate.
- Normalize within each run: Controls are on a separate plate or should be averaged across all plates. Data will be normalized across the whole run.
- Already normalized: Imported data is already normalized to % control activity.
- No controls (do not normalize): The data is not normalized and no control data is available. Raw values to be used for data calculations.
Normalization is based on positive and negative controls, as follows
|is the sample,|
|is the mean of negative controls, and|
|is the mean of positive controls.|
Normalized Dose-Response Plots
When data is normalized (first three options), we automatically fix the y-axis range of dose-response plots to 0-100 (%) range (including a small buffer).
How To Normalize Existing Data
If you already imported control data, simply edit the dose-response calculation readout definition to perform normalization and verify your plate control layouts.
If you did not import controls together with test data, you can import a separate control plate to the existing run, define the control layout for that new plate, and select the option “Normalize within each run”.
The database will fit your data again and plot normalized results.
Additional Dose-Response Improvements
- The dose or concentration readout definition values are now visible on the heat map.
- Search results on the Explore Data tab will show only matching dose-response plots.
Notable Other Enhancements
- Batch External IDs may now be edited manually after the batch is registered.
- Vault Administrators can now manage authorized IP addresses for Vault members and restrict CDD administrator access to their vault.
- Molecules can be deleted even if associated with a plate.